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KMID : 0624620100430080561
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BMB Reports
2010 Volume.43 No. 8 p.561 ~ p.566
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Enhancement of HIV-1 Tat fusion protein transduction efficiency by bog blueberry anthocyanins
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Lee Sun-Hwa
Jeong Hoon-Jae
Kim Dae-Won
Sohn Eun-Jeong
Kim Mi-Jin
Kim Duk-Soo
Kang Tae-Cheon
Lim Soon-Sung
Kang Il-Jun
Cho Sung-Woo
Lee Kil-Soo
Park Jin-Seu
Eum Won-Sik
Choi Soo-Young
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Abstract
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Though protein transduction domains (PTDs) are well known for the delivery of exogenous therapeutic proteins into living cells, the overall low efficiency of transduction is a serious obstacle. We investigated the effect of bog blueberry anthocyanins (BBA) on protein transduction efficiency and found that BBA enhanced the transduction efficiencies of Tat-SOD fusion protein into HeLa cells and mice skin. The enzymatic activities in the cells and skin tissue in the presence of BBA were markedly increased compared to controls. Further, BBA did not demonstrate any cell toxicity at various concentrations. Although the mechanism is not fully understood, we suggest that BBA might alter the conformation of the membrane, which would indicate that BBA can be used as a protein transduction enhancer for the efficient delivery of therapeutic proteins for a variety of disorders.
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KEYWORD
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Bog blueberry anthocyanins, Protein therapy, Protein transduction efficiency, Tat fusion protein
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